It’s also worth noting that even if alternatives superior to agar were found, scientists are reluctant to abandon established protocols (even when microbiologists do use other jellies, they often still add agar to the mix, for example, to increase the gel strength of the solid media). As agar has been the standard gelling agent in microbiology for around 150 years, an enormous infrastructure of standardized methods, reference values, and quality control procedures has emerged around its specific properties. Switching to a different medium (even a superior one) means results may not be directly comparable to decades of published literature or to other laboratories’ findings.
The two most popular explanations of origin are that the belief goes back to pagan times when we believed in tree spirits, or that we are invoking Christ’s protection by referring to the wood of the Cross. The former is nothing but guesswork, based on the conviction that all superstitions must be ancient, and it has the usual problem of spanning thousands of years with no evidence at all of its existence, or, for that matter, any evidence that ‘we’ ever believed in tree spirits.
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